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hek cells  (ATCC)


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    ATCC hek cells
    DISC1–PML interaction is required for NPC proliferation in the developing cortex. A, lysates from human embryonic kidney <t>(HEK)</t> <t>cells</t> co-transfected with DISC1, PML and WT IE1 or IE1-L174P were immunoprecipitated with an anti-PML antibody and immunoblotted with an anti-HA antibody. B, lysates from HEK cells cotransfected with DISC1, IE1, and WT PML or mutant PML lacking the IE1-binding site (PMLΔIE1) were immunoprecipitated with an anti-PML antibody and immunoblotted with an anti-HA antibody. C, HEK cells cotransfected with PML and HA-tagged WT DISC1, or mutant DISC1 lacking the PML-binding site (DISC1ΔPML), were immunoprecipitated with the PML antibody and immunoblotted with the HA antibody. D, mouse embryos electroporated with various constructs at E13.5 were pulse labeled with BrdU (50 mg/kg) for 2 h at E15.5. Bar graph represents the percentage of GFP- and BrdU-double positive cells over total GFP-positive cells in the VZ/SVZ. Green , cells transfected with GFP, DISC1 RNAi, and DISC1 constructs; red , BrdU-positive cells; arrowheads indicate GFP- and BrdU-double positive cells. The scale bar represents 20 μm. Graph shows mean +/− SEM (GFP: n = 4, DISC1 RNAi: n = 6, DISC1 RNAi + Wt DISC1: n = 4, DISC1 RNAi + DISC1ΔPML: n = 3, Tukey’s multiple comparison test ∗ p < 0.05; one-way ANOVA: F(3,13) = 6.067, p = 0.0082). BrdU, bromodeoxyuridine; E13.5, embryonic day 13.5; E15.5, embryonic day 15.5; HA, hemagglutinin; NPC, neural progenitor cell; IE1, immediate early 1; SZ, subventricular zone; VZ, ventricular zone; PML, promyelocytic leukemia.
    Hek Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 750 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    hek cells - by Bioz Stars, 2026-09
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    1) Product Images from "Cytomegalovirus-encoded immediate early 1 protein perturbs neural progenitor proliferation via interfering with host PML–DISC1 interaction"

    Article Title: Cytomegalovirus-encoded immediate early 1 protein perturbs neural progenitor proliferation via interfering with host PML–DISC1 interaction

    Journal: The Journal of Biological Chemistry

    doi: 10.1016/j.jbc.2026.111269

    DISC1–PML interaction is required for NPC proliferation in the developing cortex. A, lysates from human embryonic kidney (HEK) cells co-transfected with DISC1, PML and WT IE1 or IE1-L174P were immunoprecipitated with an anti-PML antibody and immunoblotted with an anti-HA antibody. B, lysates from HEK cells cotransfected with DISC1, IE1, and WT PML or mutant PML lacking the IE1-binding site (PMLΔIE1) were immunoprecipitated with an anti-PML antibody and immunoblotted with an anti-HA antibody. C, HEK cells cotransfected with PML and HA-tagged WT DISC1, or mutant DISC1 lacking the PML-binding site (DISC1ΔPML), were immunoprecipitated with the PML antibody and immunoblotted with the HA antibody. D, mouse embryos electroporated with various constructs at E13.5 were pulse labeled with BrdU (50 mg/kg) for 2 h at E15.5. Bar graph represents the percentage of GFP- and BrdU-double positive cells over total GFP-positive cells in the VZ/SVZ. Green , cells transfected with GFP, DISC1 RNAi, and DISC1 constructs; red , BrdU-positive cells; arrowheads indicate GFP- and BrdU-double positive cells. The scale bar represents 20 μm. Graph shows mean +/− SEM (GFP: n = 4, DISC1 RNAi: n = 6, DISC1 RNAi + Wt DISC1: n = 4, DISC1 RNAi + DISC1ΔPML: n = 3, Tukey’s multiple comparison test ∗ p < 0.05; one-way ANOVA: F(3,13) = 6.067, p = 0.0082). BrdU, bromodeoxyuridine; E13.5, embryonic day 13.5; E15.5, embryonic day 15.5; HA, hemagglutinin; NPC, neural progenitor cell; IE1, immediate early 1; SZ, subventricular zone; VZ, ventricular zone; PML, promyelocytic leukemia.
    Figure Legend Snippet: DISC1–PML interaction is required for NPC proliferation in the developing cortex. A, lysates from human embryonic kidney (HEK) cells co-transfected with DISC1, PML and WT IE1 or IE1-L174P were immunoprecipitated with an anti-PML antibody and immunoblotted with an anti-HA antibody. B, lysates from HEK cells cotransfected with DISC1, IE1, and WT PML or mutant PML lacking the IE1-binding site (PMLΔIE1) were immunoprecipitated with an anti-PML antibody and immunoblotted with an anti-HA antibody. C, HEK cells cotransfected with PML and HA-tagged WT DISC1, or mutant DISC1 lacking the PML-binding site (DISC1ΔPML), were immunoprecipitated with the PML antibody and immunoblotted with the HA antibody. D, mouse embryos electroporated with various constructs at E13.5 were pulse labeled with BrdU (50 mg/kg) for 2 h at E15.5. Bar graph represents the percentage of GFP- and BrdU-double positive cells over total GFP-positive cells in the VZ/SVZ. Green , cells transfected with GFP, DISC1 RNAi, and DISC1 constructs; red , BrdU-positive cells; arrowheads indicate GFP- and BrdU-double positive cells. The scale bar represents 20 μm. Graph shows mean +/− SEM (GFP: n = 4, DISC1 RNAi: n = 6, DISC1 RNAi + Wt DISC1: n = 4, DISC1 RNAi + DISC1ΔPML: n = 3, Tukey’s multiple comparison test ∗ p < 0.05; one-way ANOVA: F(3,13) = 6.067, p = 0.0082). BrdU, bromodeoxyuridine; E13.5, embryonic day 13.5; E15.5, embryonic day 15.5; HA, hemagglutinin; NPC, neural progenitor cell; IE1, immediate early 1; SZ, subventricular zone; VZ, ventricular zone; PML, promyelocytic leukemia.

    Techniques Used: Transfection, Immunoprecipitation, Mutagenesis, Binding Assay, Construct, Labeling, Comparison

    Related Articles

    other:

    Article Title: IGSF10 is a RET antagonist regulating Ewing sarcoma growth and GnRH neuron migration
    Article Snippet: A673, SK-N-MC, SK-NEP-1, SK-ES-1, RD-ES, and 293T cells were from ATCC.

    Article Title: Development of in vitro and in vivo neutralization assays for New World alphaviruses based on the VSV pseudovirus system
    Article Snippet: The cell lines used in this study included HEK293T (ATCC, CRL-3216), HEK293FT (Invitrogen, Carlsbad, CA, USA, R70007 ), HepG2 (ATCC, HB-8065), Vero (ATCC, CCL81), A549 (ATCC, CCL-185), HeLa (ATCC, CCL-2), BHK21(ATCC, CCL-10), K562 (ATCC, CCL-243), SK-N-MC (ATCC, HTB-10), CHO (ATCC, CRL-3440), Hep2 (ATCC,CCL-23), Huh7 (Japanese Collection of Research Bioresources [JCRB], 0403).

    Article Title: Development of in vitro and in vivo neutralization assays for New World alphaviruses based on the VSV pseudovirus system.
    Article Snippet: The cell lines used in this study included HEK293T (ATCC, CRL-3216), HEK293FT (Invitrogen, Carlsbad, CA, USA, R70007), HepG2 (ATCC, HB-8065), Vero (ATCC, CCL81), A549 (ATCC, CCL-185), HeLa (ATCC, CCL-2), BHK21(ATCC, CCL-10), K562 (ATCC, CCL-243), SK-N-MC (ATCC, HTB-10), CHO (ATCC, CRL-3440), Hep2 (ATCC,CCL-23), Huh7 (Japanese Collection of Research Bioresources [JCRB], 0403).

    Cell Culture:

    Article Title: Global Profiling of Remodeled Subcellular Structures Due to Drug Treatment and Disease
    Article Snippet: .. Cell lines A673 (ATCC, CRL-1598), SK-N-MC (ATCC, HTB-10), U2OS (ATCC, HTB-96), and HEK293T/17 (ATCC, CRL-11268) were obtained from ATCC and cultured at 5% CO 2 and 37 oC. ..

    Article Title: Diverse oncogenes use common mechanisms to drive growth of major forms of human cancer.
    Article Snippet: HAP1 (#C631, RRID:CVCL_Y019) and KMB- 7 (#C628, RRID:CVCL_A426) cell lines were obtained from Horizon Discovery and maintained in low- density cultures in Iscove’s modified Dulbecco’s medium according to the vendor’s guidelines. .. RKO (RRID:CVCL_ 0504), HCT116 (RRID:CVCL_0291), LoVo (RRID:CVCL_0399), BT- 474 (RRID:CVCL_0179), T47D (RRID:CVCL_0553), MCF- 7 (RRID:CVCL_0031), NCI- H1975 (RRID:CVCL_1511), A549 (RRID: CVCL_0023), K562 (RRID:CVCL_0004), CRL- 2061 (RRID:CVCL_0041), VCaP (RRID:CVCL_2235), and SK- N- MC (RRID:CVCL_0530) cells were purchased from American Type Culture Collection and cultured according to the vendor’s guidelines in the medium specified by the vendor. ..

    Control:

    Article Title: Systematic profiling reveals distinct senescence signatures and regulators across human brain cell types.
    Article Snippet: .. We treated with 100 μM BrdU25 or a DMSO control for 7 days in SVG-A (astrocytes), HBEC-5i (endothelial cells), HMC3 (microglia), HOG (oligodendrocytes), and SK-N-MC (neurons with some DA-like features, such as moderate dopamine-beta-hydroxylase activity (ATCC)) lines and analyzed various well-established hallmarks of a senescence phenotype (Figs. 1A and S1A). ..

    Article Title: Systematic profiling reveals distinct senescence signatures and regulators across human brain cell types
    Article Snippet: .. We treated with 100 μM BrdU or a DMSO control for 7 days in SVG-A (astrocytes), HBEC-5i (endothelial cells), HMC3 (microglia), HOG (oligodendrocytes), and SK-N-MC (neurons with some DA-like features, such as moderate dopamine-beta-hydroxylase activity (ATCC)) lines and analyzed various well-established hallmarks of a senescence phenotype (Figs. and ). ..

    Activity Assay:

    Article Title: Systematic profiling reveals distinct senescence signatures and regulators across human brain cell types.
    Article Snippet: .. We treated with 100 μM BrdU25 or a DMSO control for 7 days in SVG-A (astrocytes), HBEC-5i (endothelial cells), HMC3 (microglia), HOG (oligodendrocytes), and SK-N-MC (neurons with some DA-like features, such as moderate dopamine-beta-hydroxylase activity (ATCC)) lines and analyzed various well-established hallmarks of a senescence phenotype (Figs. 1A and S1A). ..

    Article Title: Systematic profiling reveals distinct senescence signatures and regulators across human brain cell types
    Article Snippet: .. We treated with 100 μM BrdU or a DMSO control for 7 days in SVG-A (astrocytes), HBEC-5i (endothelial cells), HMC3 (microglia), HOG (oligodendrocytes), and SK-N-MC (neurons with some DA-like features, such as moderate dopamine-beta-hydroxylase activity (ATCC)) lines and analyzed various well-established hallmarks of a senescence phenotype (Figs. and ). ..



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    Korean Cell Line Bank sk n mc cells
    DISC1–PML interaction is required for NPC proliferation in the developing cortex. A, lysates from human embryonic kidney <t>(HEK)</t> <t>cells</t> co-transfected with DISC1, PML and WT IE1 or IE1-L174P were immunoprecipitated with an anti-PML antibody and immunoblotted with an anti-HA antibody. B, lysates from HEK cells cotransfected with DISC1, IE1, and WT PML or mutant PML lacking the IE1-binding site (PMLΔIE1) were immunoprecipitated with an anti-PML antibody and immunoblotted with an anti-HA antibody. C, HEK cells cotransfected with PML and HA-tagged WT DISC1, or mutant DISC1 lacking the PML-binding site (DISC1ΔPML), were immunoprecipitated with the PML antibody and immunoblotted with the HA antibody. D, mouse embryos electroporated with various constructs at E13.5 were pulse labeled with BrdU (50 mg/kg) for 2 h at E15.5. Bar graph represents the percentage of GFP- and BrdU-double positive cells over total GFP-positive cells in the VZ/SVZ. Green , cells transfected with GFP, DISC1 RNAi, and DISC1 constructs; red , BrdU-positive cells; arrowheads indicate GFP- and BrdU-double positive cells. The scale bar represents 20 μm. Graph shows mean +/− SEM (GFP: n = 4, DISC1 RNAi: n = 6, DISC1 RNAi + Wt DISC1: n = 4, DISC1 RNAi + DISC1ΔPML: n = 3, Tukey’s multiple comparison test ∗ p < 0.05; one-way ANOVA: F(3,13) = 6.067, p = 0.0082). BrdU, bromodeoxyuridine; E13.5, embryonic day 13.5; E15.5, embryonic day 15.5; HA, hemagglutinin; NPC, neural progenitor cell; IE1, immediate early 1; SZ, subventricular zone; VZ, ventricular zone; PML, promyelocytic leukemia.
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    DISC1–PML interaction is required for NPC proliferation in the developing cortex. A, lysates from human embryonic kidney (HEK) cells co-transfected with DISC1, PML and WT IE1 or IE1-L174P were immunoprecipitated with an anti-PML antibody and immunoblotted with an anti-HA antibody. B, lysates from HEK cells cotransfected with DISC1, IE1, and WT PML or mutant PML lacking the IE1-binding site (PMLΔIE1) were immunoprecipitated with an anti-PML antibody and immunoblotted with an anti-HA antibody. C, HEK cells cotransfected with PML and HA-tagged WT DISC1, or mutant DISC1 lacking the PML-binding site (DISC1ΔPML), were immunoprecipitated with the PML antibody and immunoblotted with the HA antibody. D, mouse embryos electroporated with various constructs at E13.5 were pulse labeled with BrdU (50 mg/kg) for 2 h at E15.5. Bar graph represents the percentage of GFP- and BrdU-double positive cells over total GFP-positive cells in the VZ/SVZ. Green , cells transfected with GFP, DISC1 RNAi, and DISC1 constructs; red , BrdU-positive cells; arrowheads indicate GFP- and BrdU-double positive cells. The scale bar represents 20 μm. Graph shows mean +/− SEM (GFP: n = 4, DISC1 RNAi: n = 6, DISC1 RNAi + Wt DISC1: n = 4, DISC1 RNAi + DISC1ΔPML: n = 3, Tukey’s multiple comparison test ∗ p < 0.05; one-way ANOVA: F(3,13) = 6.067, p = 0.0082). BrdU, bromodeoxyuridine; E13.5, embryonic day 13.5; E15.5, embryonic day 15.5; HA, hemagglutinin; NPC, neural progenitor cell; IE1, immediate early 1; SZ, subventricular zone; VZ, ventricular zone; PML, promyelocytic leukemia.

    Journal: The Journal of Biological Chemistry

    Article Title: Cytomegalovirus-encoded immediate early 1 protein perturbs neural progenitor proliferation via interfering with host PML–DISC1 interaction

    doi: 10.1016/j.jbc.2026.111269

    Figure Lengend Snippet: DISC1–PML interaction is required for NPC proliferation in the developing cortex. A, lysates from human embryonic kidney (HEK) cells co-transfected with DISC1, PML and WT IE1 or IE1-L174P were immunoprecipitated with an anti-PML antibody and immunoblotted with an anti-HA antibody. B, lysates from HEK cells cotransfected with DISC1, IE1, and WT PML or mutant PML lacking the IE1-binding site (PMLΔIE1) were immunoprecipitated with an anti-PML antibody and immunoblotted with an anti-HA antibody. C, HEK cells cotransfected with PML and HA-tagged WT DISC1, or mutant DISC1 lacking the PML-binding site (DISC1ΔPML), were immunoprecipitated with the PML antibody and immunoblotted with the HA antibody. D, mouse embryos electroporated with various constructs at E13.5 were pulse labeled with BrdU (50 mg/kg) for 2 h at E15.5. Bar graph represents the percentage of GFP- and BrdU-double positive cells over total GFP-positive cells in the VZ/SVZ. Green , cells transfected with GFP, DISC1 RNAi, and DISC1 constructs; red , BrdU-positive cells; arrowheads indicate GFP- and BrdU-double positive cells. The scale bar represents 20 μm. Graph shows mean +/− SEM (GFP: n = 4, DISC1 RNAi: n = 6, DISC1 RNAi + Wt DISC1: n = 4, DISC1 RNAi + DISC1ΔPML: n = 3, Tukey’s multiple comparison test ∗ p < 0.05; one-way ANOVA: F(3,13) = 6.067, p = 0.0082). BrdU, bromodeoxyuridine; E13.5, embryonic day 13.5; E15.5, embryonic day 15.5; HA, hemagglutinin; NPC, neural progenitor cell; IE1, immediate early 1; SZ, subventricular zone; VZ, ventricular zone; PML, promyelocytic leukemia.

    Article Snippet: SK-N-MC human neuroblastoma cells and HEK cells were purchased from ATCC.

    Techniques: Transfection, Immunoprecipitation, Mutagenesis, Binding Assay, Construct, Labeling, Comparison